Application

Research CategoryProtein Trafficking

Detect Ubiquitin using this Anti-Ubiquitin Antibody validated for use in WB, IH.

Immunohistochemistry: 1:2,000-1:5,000.

Immunoblotting: 1:1,000-1:2,000 (depending on detection system used).

Optimal working dilutions must be determined by end user.

Research Sub CategoryUbiquitin & Ubiquitin Metabolism

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Ubiquitin from bovine red blood cells coupled to chicken gamma globulin.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-454

Physical form

Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 mL of sterile distilled water. Centrifuge to remove any residue.

Specificity

Recognizes both free ubiquitin and ubiquitin-protein conjugates on Western blots. Labels tangles and Lewy bodies in paraffin or frozen human brain sections.

Storage and Stability

Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.